Utilizing a Reconfigurable Maze System to Enhance the Reproducibility of Spatial Navigation Tests in Rodents

Several maze shapes are used to test spatial navigation performance and behavioral phenotypes. Traditionally, each experiment requires a unique shape, thus requiring several separate mazes in different configurations. The geometry cannot be reconfigured single environment accommodate scalability reproducibility. reconfigurable is approach address the limitations, allowing quick flexible configurations of pathways repeatable manner. It consists interlocking includes feeders, treadmills, movable walls, shut-off sensors. current protocol describes how can replicate existing mazes, including T-shaped, plus-shaped, W-shaped, figure-eight mazes. Initially, T-shaped was constructed inside experimental room, followed by modifications. rapid scalable outlined herein demonstrates flexibility maze, achieved through addition components training phases stepwise systematically precisely assesses multiple aspects behavior.